Journal: Cell reports

Article Title: The exocyst subunit EXOC2 regulates the toxicity of expanded GGGGCC repeats in C9ORF72 -ALS/FTD

doi: 10.1016/j.celrep.2024.114375

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit anti-NMDAR 2B , Alomone Labs , Cat#AGC-003.

Techniques: Reverse Transcription, Enzyme-linked Immunosorbent Assay, Control, Recombinant, Plasmid Preparation, Software

Journal: Marine Drugs

Article Title: α-Conotoxin TxIB Reversed Nicotine-Induced Locomotor Sensitization and Nicotine-Enhanced Dopaminergic Activity in Mice

doi: 10.3390/md23030109

Figure Lengend Snippet: Figure 6. α-CTx TxIB suppressed the expressions of NMDAR2B/DAT and c-Fos in the VTA and NAc of mice induced by nicotine. Data represent mean ± S.E.M. for 3–9 mice. The red columns represent the control group, and # represents the data for each individual mouse in this group. The yellow columns represent the nicotine-treated group, and □represents the data for each individual mouse in this group. The blue columns represent the 5 nmol TxIB administration group, and 3 represents the data for each individual mouse in this group. (A) The expressions of NMDAR/SERT/DAT/c- Fos/GAPDH in NAc/PFC/CPU/HIP analyzed by Western blotting; (B) The densities relative to GAPDH of NMDAR/DAT and c-Fos in NAc/PFC/CPU/HIP. * denotes a significant difference from the control group; # denotes a significant difference from the nicotine-treated group (* p < 0.05, ** p < 0.01, *** p < 0.001, # p < 0.05, ## p < 0.01, ### p < 0.001).

Article Snippet: Membranes were blocked with 5% skim milk in TBST (Tris-buffered saline with 0.1% Tween-20) for 30 min at room temperature, then incubated overnight at 4 ◦C with primary antibodies against NMDAR-2B (21920-1-AP, Proteintech, Wuhan, China), SERT (19559-1-AP, Proteintech), DAT (22524-1-AP, Proteintech), c-Fos (ABE457, Sigma-Aldrich, St. Louis, MO, USA), and GAPDH (10494-1-AP, Proteintech) diluted in TBST containing 3% BSA.

Techniques: Control, Western Blot

Journal: Brain Structure & Function

Article Title: Expression of synaptic proteins and development of dendritic spines in fetal and postnatal neocortex of the pig, the European wild boar Sus scrofa

doi: 10.1007/s00429-025-02900-0

Figure Lengend Snippet: Antibodies and reagents for staining and protein blotting

Article Snippet: NMDAR subunit 2B (NR2B/GluN2B) , Rabbit, WB , Millipore, Burlington, MA, USA, Cat# 06-600, RRID: AB_310193 , 1:1000.

Techniques: Staining

Journal: Brain Structure & Function

Article Title: Expression of synaptic proteins and development of dendritic spines in fetal and postnatal neocortex of the pig, the European wild boar Sus scrofa

doi: 10.1007/s00429-025-02900-0

Figure Lengend Snippet: Developmental expression of plasticity-related proteins. ( A ) NMDAR subunit GluN1. ( B ) NMDAR subunit GluN2B. ( C ) Postsynaptic enzyme CamKIIα, total protein. ( D ) T286 phosphorylated CamKIIα. ( E ) Structural plasticity-related actin bundling protein α-actinin-2. ( F ) Spine apparatus and cisternal organelle-associated synaptopodin. β-actin at 42 kDa was used for normalization. n.m., the protein was not measurable at this age in this cortex

Article Snippet: NMDAR subunit 2B (NR2B/GluN2B) , Rabbit, WB , Millipore, Burlington, MA, USA, Cat# 06-600, RRID: AB_310193 , 1:1000.

Techniques: Expressing

Journal: NPJ Science of Food

Article Title: Dietary methionine supplementation improves cognitive dysfunction associated with transsulfuration pathway upregulation in subacute aging mice

doi: 10.1038/s41538-024-00348-w

Figure Lengend Snippet: A AGEs, B lipofuscin, and C Aβ-40 levels in the hippocampi, D the relative mRNA expression levels of NF-κB, Nrf2, HO-1, NQO-1, Bcl-2, caspase3, and Bax in the hippocampi, E the relative mRNA expression levels of BDNF, TrkB, RC3, Gap-43, PSD-95, and SYNAPO in the hippocampi, F the relative mRNA expression levels of Nr1, Nr2a, Nr2b, CAMK2A, CAMK2B, and CAMK2D in the hippocampi, G , H the protein expression levels of P-NF-κB p65, NF-κB p65, BDNF, Nr2b, P-CAMK2, and CAMK2 in the hippocampi. ND: saline + normal diet; D + ND: D-Gal + normal diet; D + MS: D-Gal + Met supplementation. Data are represented as mean ± SEM ( n = 8 or 6 or 3). “ns” means not significantly different; * p < 0.05, ** p < 0.01, *** p < 0.001, versus ND; # p < 0.05, ## p < 0.01, ### p < 0.001, versus D + ND.

Article Snippet: N-methyl-D-aspartate receptor (NMDAR) 2b (Nr2b, product code GB115472) and CBS (product code GB111794) were purchased from Servicebio (Wuhan, China).

Techniques: Expressing, Saline